1. Specimen Management Bench - Monday to Friday :
a) Accessioning and processing all Virology specimens.
b) For PCR testing: mix well and transfer CSF/ EDTA blood / Amniotic fluid to Blue-topped vial (e.g. HSV, EBV, CMV, Parvovirus B19, Polyomavirus, Enterovirus.)
b) Preparing and staining of Direct Smears, PCP slides.
c) Send out stool or Urine for EM.http://bahankuliahkesehatan.blogspot.com/
2. Shell Vial Bench – Monday to Friday :
a) Staining and reading of all shell vials.
b) Reading of all Direct Smears and PCP slides.
c) Read Super E-mix Shell vials daily.
d) Enter all shell vial related (e.g. new reagent lots, daily staining, weekly viral propagation and MRC-5 growth) QC results either in LIS and/or Data Sheet.
e) Tabulate and fax weekly viral isolates data (on/after Friday).
f) Maintain stocks of viral isolates as well as preparation of QC slide.
3. CMV Antigenemia Bench :
a) Monday to Friday: Record temperatures and humidity on freezers, fridges and room every morning.
b) Monday to Friday: Process, stain and read all CMV antigenemia specimens.
c) Perform RPR on Wednesday to Friday.
d) Perform VZ Ab testing using VIDAS.
e) Perform Monospot daily.
4. Molecular Bench:
a) Perform Tagman HCV RNA PCR using Ampliprep when there
are enough of specimens for a full run.
b) Perform Tagman HBV DNA Assay using Ampliprep when there are enough specimens for a full run.
c) Assist PCR bench with Chlamydia/GC testing.
d) Perform molecular bench inventories.
5. AXSYM Bench:
a) Perform all stat Hepatitis markers, HIV1&2, CMV IgG and Rubella
IgG and HIV assays and QC’s using AxSYM.
b) Perform weekly maintenance on Thursday or Friday.
c) Perform monthly maintenance at beginning of each month.
d) Perform RPR (syphilis) on Monday and Tuesday.
e) Do inventory on Wednesday/Thursday.
6. Eye Bank bench: Using NEXGEN:
a) Perform Eye Bank -BioRad EIA HBsAg &HIV and Ortho EIA HBcAb & HCV Ab daily.
b) Perform C. difficile Toxin A/B testing daily.
c) Perform EBV VCA IgG once a week.
7. PCR Bench :
a) Perform Chlamydia/GC using ProbTec.
b) Perform WNV RT-PCR for TGLN and CSF specimens; freeze samples and enter into LIS store.
d) Perform RNA and DNA extractions from CSF/whole blood.
e) Perform HSV PCR Tuesday and Friday.
f) Perform EBV PCR,CMV PCR and Parvo B19 PCR once a week.
g) Perform Enterovirus PCR when received.
8. P-Lab Bench :
a) Perform Ortho HTLV 1&2 Ab
b) Perform Immucor CMV Ab
c) Perform WNV IgM Ab
d) Freeze all transplant patients sera in freezer program under ‘Donor’, Inception Biosciences sera under ‘CTSCP’ and Cord Blood of Canada sera under ‘CBB’.
9. Evening Technologist:
a) Perform stat TGLN / stat recipient assays if requested.
b) Perform WNV IgM assay
c) Perform AxSYM assays, QC’s and maintenance
d) Process time-sensitive specimens (eg.molecular tests).
10. Night Technologist:
a) Perform stat TGLN / stat recipient assays if requested.
b) Continue WNV IgM assay if set up late.
c) Perform AxSYM assays, QC’s and maintenance.
d) Process time-sensitive specimens (eg.molecular tests).
11. Technician 1- Monday to Friday :
a) Disinfect, check centrifuges and document on sheet.
b) Spin and accession all Serology specimens.
c) Send out all PHL specimens and printing out manifest.
d) Process Viral load specimens.
e) Document pressure reading in LIS for Serology Accession Hood.
12. Technician 2- Monday to Friday :
a) Disinfect, check centrifuges and document on sheet if not yet done by Technician 1.
b) Process Viral load specimens.
c) Enter patients’ demographic in Viral Load program.
d) Spin, accession and freeze sera for HBV DNA and HCV RNA.
Help technician 2 to spin and accession all serology specimens
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